International Journal of Antimicrobial Agents
Volume 23, Issue 6 , Pages 606-612 , June 2004

Reduction in the bactericidal activity of selected cathelicidin peptides by bovine calf serum or exogenous endotoxin

  • Karen H Bartlett

      Affiliations

    • Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, 100 Oakdale Campus, IREH, Iowa City, IA 52242, USA
    • Present address: School of Occupational and Environmental Hygiene, University of British Columbia, 2206 East Mall, Vancouver, BC, Canada V6T 1Z3. Tel.: +1-604-822-6019.
    • ,
  • Paul B McCray Jr.

      Affiliations

    • Department of Pediatrics, University of Iowa, 2544 JCP, Iowa City, IA 52242-5000, USA
    • Tel.: +1-319-356-4866.
    • ,
  • Peter S Thorne

      Affiliations

    • Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, 100 Oakdale Campus, IREH, Iowa City, IA 52242, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-319-335-4216; fax: +1-319-335-4006.

,Accepted 26 February 2004.

References 

  1. Saiman L, Tabibi S, Starner TD, et al.  Cathelicidin peptides inhibit multiply antibiotic-resistant pathogens from patients with cystic fibrosis. Antimicrob Agents Chemother. 2001;45:2838–2844
  2. Travis SM, Anderson NN, Forsyth WR, et al.  Bactericidal activity of mammalian cathelicidin-derived peptides. Infect Immun. 2000;68:2748–2755
  3. Travis SM, Conway BA, Zabner J, et al.  Activity of abundant antimicrobials of the human airway. Am J Respir Cell Mol Biol. 1999;20:872–879
  4. Zanetti M, Gennaro R, Romeo D. Cathelicidins: a novel protein family with a common proregion and a variable C-terminal antimicrobial domain. FEBS Lett. 1995;374:1–5
  5. Tossi A, Scocchi M, Skerlavaj B, Gennaro R. Identification and characterization of a primary antibacterial domain CAP18, a lipopolysaccharide binding protein from rabbit leukocytes. FEBS Lett. 1994;339:108–112
  6. Zanetti M, Gennaro R, Scocchi M, Skerlavaj B. Structure and biology of cathelicidins. Adv Exp Med Biol. 2000;479:203–218
  7. Hirata M, Zhong J, Wright SC, Larrick JW. Structure and functions of endotoxin-binding peptides derived from CAP18. Prog. Clin. Biol. Res. 1995;392:317–326
  8. Hirata M, Shimomura Y, Yoshida M, et al.  Characterization of a rabbit cationic protein (CAP18) with lipopolysaccharide-inhibitory activity. Infect Immun. 1994;62:1421–1426
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  10. Scott MG, Vreugdenhil ACE, Buurman WA, Hancock REW, Gold MR. Cutting edge: cationic antimicrobial peptides block the binding of lipopolysaccharide (LPS) to LPS binding protein. J. Immunol. 2000;164:549–553
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  12. Kirikae T, Hirata M, Yamasu H, et al.  Protective effects of a human 19-kilodalton cationic antimicrobial protein (CAP18)-derived peptide against murine endotoxemia. Infect. Immun. 1998;66:1861–1868
  13. Bagella L, Scocchi M, Zanetti M. cDNA sequences of three sheep myeloid cathelicidins. FEBS Lett. 1995;376:225–228
  14. Brogden KA, Kalfa VC, Ackermann MR, Palmquist DE, McCray PB, Tack BF. The ovine cathelicidin SMAP29 kills ovine respiratory pathogens in vitro and in an ovine model of pulmonary infection. Antimicrob Agents Chemother. 2001;45:331–334
  15. Kalfa VC, Jia HP, Kunkle RA, McCray PB, Tack BF, Brogden KA. Congeners of SMAP29 kill ovine pathogens and induce ultrastructure damage in bacterial cells. Antimicrob Agents Chemother. 2001;45:3256–3261
  16. Sawai MV, Waring AJ, Kearney WR, et al.  Impact of single-residue mutations on the structure and function of ovispirin/novispirin antimicrobial peptides. Protein Eng. 2002;15:225–232
  17. Steinstraesser L, Tack BF, Waring AJ, et al.  Activity of novispirin G10 against Pseudomonas aeruginosa in vitro and in infected burns. Antimicrob Agents Chemother. 2002;46:1837–1844
  18. Tack BF, Sawai MV, Wret K, et al.  SMAP-29 has two LPS-binding sites and a central hinge. Eur J Biochem. 2002;269:1181–1189
  19. Thorne PS. Inhalation toxicology models of endotoxin- and bioaerosol-induced inflammation. Toxicology. 2000;152:13–23
  20. Steinberg DA, Lehrer RI. Designer assays for antimicrobial peptides. Methods Mol Biol. 1997;78:169–186
  21. Skerlavaj B, Benincasa M, Risso A, Zanetti M, Gennaro R. SMAP-29: a potent antibacterial and antifungal peptide from sheep leukocytes. FEBS Lett. 1999;463:58–62

PII: S0924-8579(04)00097-4

doi: 10.1016/j.ijantimicag.2004.02.018

International Journal of Antimicrobial Agents
Volume 23, Issue 6 , Pages 606-612 , June 2004

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