In vivo efficacy of sitafloxacin in a new murine model of non-typeable Haemophilus influenzae pneumonia by sterile intratracheal tube

Nakamura, Shigeki; Yanagihara, Katsunori; Araki, Nobuko; Morinaga, Yoshitomo; Izumikawa, Koichi; Seki, Masafumi; Kakeya, Hiroshi; Yamamoto, Yoshihiro; Kamihira, Shimeru; Kohno, Shigeru

doi:10.1016/j.ijantimicag.2009.03.011

« Previous Next »International Journal of Antimicrobial Agents
Volume 34, Issue 3 , Pages 210-214, September 2009

In vivo efficacy of sitafloxacin in a new murine model of non-typeable Haemophilus influenzae pneumonia by sterile intratracheal tube

Shigeki Nakamura
- Second Department of Internal Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
- These authors contributed equally to this work.
Katsunori Yanagihara
- Second Department of Internal Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
- Department of Laboratory Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
- These authors contributed equally to this work.
- Corresponding author. Present address: Department of Laboratory Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. Tel.: +81 958 197 276; fax: +81 958 497 285.
Nobuko Araki
- Department of Laboratory Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
Yoshitomo Morinaga
- Second Department of Internal Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
- Department of Laboratory Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
Koichi Izumikawa
- Second Department of Internal Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
Masafumi Seki
- Second Department of Internal Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
Hiroshi Kakeya
- Second Department of Internal Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
Yoshihiro Yamamoto
- Second Department of Internal Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
Shimeru Kamihira
- Department of Laboratory Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
Shigeru Kohno
- Second Department of Internal Medicine, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan
- Division of Molecular & Clinical Microbiology, Department of Molecular Microbiology & Immunology, Nagasaki University Graduate School of Medical Science, Nagasaki, Japan

Received 5 January 2009; accepted 15 March 2009. published online 27 April 2009.

Abstract

A novel murine model of non-typeable Haemophilus influenzae (NTHi) pneumonia was established. A plastic tube was inserted into the trachea 7 days before bacterial inoculation. Numbers of NTHi recovered from the lungs and trachea were determined for 7 days. Histologically, bronchioles and adjacent alveoli in the intubation group were filled with numerous inflammatory cells. The efficacy of sitafloxacin was compared with ciprofloxacin using the new murine pneumonia model. The data suggest that sitafloxacin displays equivalent efficacy to ciprofloxacin against H. influenzae pneumonia. This new murine NTHi pneumonia model appears useful not only for in vivo evaluation of antibiotics but also for analysis of the pathogenesis of H. influenzae pneumonia.

Keywords: Haemophilus influenzae, Non-typeable, Sterile intratracheal tube, Sitafloxacin