Carriage of genes for various extended-spectrum β-lactamases: a novel resistance strategy of Klebsiella pneumoniae in Poland

Dzierżanowska, Danuta; Kamińska, Wanda; Semczuk, Katarzyna; Borowiec, Dariusz; Matysiak, Michał; Szumała-Kąkol, Anna; Gierczyński, Rafał; Patzer, Jan A.

doi:10.1016/j.ijantimicag.2009.12.010

« Previous Next »International Journal of Antimicrobial Agents
Volume 35, Issue 4 , Pages 392-395, April 2010

Carriage of genes for various extended-spectrum β-lactamases: a novel resistance strategy of Klebsiella pneumoniae in Poland

Danuta Dzierżanowska
- Department of Clinical Microbiology and Immunology, Children's Memorial Health Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland
Wanda Kamińska
- Department of Clinical Microbiology and Immunology, Children's Memorial Health Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland
Katarzyna Semczuk
- Department of Clinical Microbiology and Immunology, Children's Memorial Health Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland
Dariusz Borowiec
- Department of Clinical Microbiology and Immunology, Children's Memorial Health Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland
Michał Matysiak
- Clinic of Paediatrics, Haematology and Oncology, Warsaw Medical University, ul. Marszałkowska 24, 00-576 Warsaw, Poland
Anna Szumała-Kąkol
- Bacteriological Laboratory, Clinical Gynaecological Hospital, Poznań University of Medical Sciences, ul. Polna 33, 60-535 Poznań, Poland
Rafał Gierczyński
- Department of Bacteriology, National Institute of Public Health–National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland
Jan A. Patzer
- Department of Clinical Microbiology and Immunology, Children's Memorial Health Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland
- Corresponding author. Tel.: +48 22 815 7271; fax: +48 22 815 7275.

Received 7 September 2009; accepted 4 December 2009. published online 08 February 2010.

Abstract

Among 110 randomly sampled strains from a collection of 247 extended-spectrum β-lactamase (ESBL)-producing clinical isolates of Klebsiella pneumoniae collected from hospitalised children in three paediatric hospitals in Poland, 64 strains (58.2%) with multiple ESBLs were found, including five non-clonal strains (4.5%) harbouring bla genes for ESBLs of three families (CTX-M, SHV and TEM). This is the first report of the emergence of triple ESBL-producing K. pneumoniae in Poland. In addition, K. pneumoniae strains harbouring bla genes for TEM-130 and TEM-132 ESBLs were detected in Poland for the first time. Epidemiological analysis of the multiple ESBL-producing K. pneumoniae isolates by pulsed-field gel electrophoresis (PFGE) revealed a relatively high genetic diversity between isolates producing the same combination of enzymes. Clonally related strains were uncommon.

Keywords: Klebsiella pneumoniae, Paediatric hospital, Multiple extended-spectrum β-lactamases, ESBLs, Epidemiology, PFGE