International Journal of Antimicrobial Agents
Volume 35, Issue 4 , Pages 342-346 , April 2010

Mutation frequency in antibiotic-resistant and -susceptible isolates of Streptococcus pneumoniae

  • Stephanie K. Henderson-Begg

      Affiliations

    • Queen Mary University of London, Barts and The London School of Medicine and Dentistry, Centre for Immunology and Infectious Disease, Blizard Institute of Cell and Molecular Science, 4 Newark Street, London E1 2AT, UK
    • Present address: University of East London, School of Health & Biosciences, Romford Road, Stratford, London E15 4LZ, UK.
  • ,
  • Carmen L. Sheppard

      Affiliations

    • Respiratory and Systemic Infection Laboratory, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
  • ,
  • Robert C. George

      Affiliations

    • Respiratory and Systemic Infection Laboratory, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
  • ,
  • David M. Livermore

      Affiliations

    • Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
  • ,
  • Lucinda M.C. Hall

      Affiliations

    • Queen Mary University of London, Barts and The London School of Medicine and Dentistry, Centre for Immunology and Infectious Disease, Blizard Institute of Cell and Molecular Science, 4 Newark Street, London E1 2AT, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 207 882 2323; fax: +44 207 882 2181.

Received 19 November 2009 ,Accepted 18 December 2009.

References 

  1. Miller K, O’Neill AJ, Chopra I. Response of Escherichia coli hypermutators to selection pressure with antimicrobial agents from different classes. J Antimicrob Chemother. 2002;49:925–934
  2. Morosini MI, Baquero MR, Sanchez-Romero JM, Negri MC, Galan JC, del Campo R, et al. Frequency of mutation to rifampin resistance in Streptococcus pneumoniae clinical strains: hexA and hexB polymorphisms do not account for hypermutation. Antimicrob Agents Chemother. 2003;47:1464–1467
  3. O’Neill AJ, Chopra I. Insertional inactivation of mutS in Staphylococcus aureus reveals potential for elevated mutation frequencies, although the prevalence of mutators in clinical isolates is low. J Antimicrob Chemother. 2002;50:161–169
  4. Tanabe K, Kondo T, Onodera Y, Furusawa M. A conspicuous adaptability to antibiotics in the Escherichia coli mutator strain, dnaQ49. FEMS Microbiol Lett. 1999;176:191–196
  5. Matic I, Radman M, Taddei F, Picard B, Doit C, Bingen E, et al. Highly variable mutation rates in commensal and pathogenic Escherichia coli. Science. 1997;277:1833–1834
  6. Oliver A, Canton R, Campo P, Baquero F, Blazquez J. High frequency of hypermutable Pseudomonas aeruginosa in cystic fibrosis lung infection. Science. 2000;288:1251–1254
  7. Richardson AR, Yu Z, Popovic T, Stojiljkovic I. Mutator clones of Neisseria meningitidis in epidemic serogroup A disease. Proc Natl Acad Sci USA. 2002;99:6103–6107
  8. Watson ME, Burns JL, Smith AL. Hypermutable Haemophilus influenzae with mutations in mutS are found in cystic fibrosis sputum. Microbiology. 2004;150:2947–2958
  9. Prunier AL, Malbruny B, Laurans M, Brouard J, Duhamel JF, Leclercq R. High rate of macrolide resistance in Staphylococcus aureus strains from patients with cystic fibrosis reveals high proportions of hypermutable strains. J Infect Dis. 2003;187:1709–1716
  10. Matic I, Radman M, Rayssiguier C. Structure of recombinants from conjugational crosses between Escherichia coli donor and mismatch-repair deficient Salmonella typhimurium recipients. Genetics. 1994;136:17–26
  11. Rayssiguier C, Thaler DS, Radman M. The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature. 1989;342:396–401
  12. Tiraby G, Sicard MA. Integration efficiencies of spontaneous mutant alleles of amiA locus in pneumococcal transformation. J Bacteriol. 1973;116:1130–1135
  13. Tiraby JG, Fox MS. Marker discrimination in transformation and mutation of pneumococcus. Proc Natl Acad Sci USA. 1973;70:3541–3545
  14. Fluit AC, Schmitz FJ, Verhoef J. Multi-resistance to antimicrobial agents for the ten most frequently isolated bacterial pathogens. Int J Antimicrob Agents. 2001;18:147–160
  15. Jones ME, Blosser-Middleton RS, Critchley IA, Thornsberry C, Sahm DF, Yamakita J, et al. The activity of levofloxacin and comparator agents against clinical isolates of Streptococcus pneumoniae collected worldwide during 1999 and 2000. Chemotherapy. 2002;48:232–237
  16. Roussel-Delvallez M, Chardon H, Dupont MJ, Fosse T, Fauchere JL, Laurans G, et al. Change of pneumococcal resistance to antibiotics in adults between 1995 and 1997: a study in eight French counties. Int J Antimicrob Agents. 2002;19:389–396
  17. Thornsberry C, Ogilvie PT, Holley HP, Sahm DF. In vitro activity of grepafloxacin and 25 other antimicrobial agents against Streptococcus pneumoniae: correlation with penicillin resistance. Clin Ther. 1998;20:1179–1190
  18. Dowson CG, Hutchison A, Brannigan JA, George RC, Hansman D, Linares J, et al. Horizontal transfer of penicillin-binding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae. Proc Natl Acad Sci USA. 1989;86:8842–8846
  19. Adrian PV, Klugman KP. Mutations in the dihydrofolate reductase gene of trimethoprim-resistant isolates of Streptococcus pneumoniae. Antimicrob Agents Chemother. 1997;41:2406–2413
  20. Maskell JP, Sefton AM, Hall LM. Mechanism of sulfonamide resistance in clinical isolates of Streptococcus pneumoniae. Antimicrob Agents Chemother. 1997;41:2121–2126
  21. del Campo R, Morosini MI, de la Pedrosa EG, Fenoll A, Munoz-Almagro C, Maiz L, et al. Population structure, antimicrobial resistance, and mutation frequencies of Streptococcus pneumoniae isolates from cystic fibrosis patients. J Clin Microbiol. 2005;43:2207–2214
  22. Gould CV, Sniegowski PD, Shchepetov M, Metlay JP, Weiser JN. Identifying mutator phenotypes among fluoroquinolone-resistant strains of Streptococcus pneumoniae using fluctuation analysis. Antimicrob Agents Chemother. 2007;51:3225–3229
  23. Hall LM, Whiley RA, Duke B, George RC, Efstratiou A. Genetic relatedness within and between serotypes of Streptococcus pneumoniae from the United Kingdom: analysis of multilocus enzyme electrophoresis, pulsed-field gel electrophoresis, and antimicrobial resistance patterns. J Clin Microbiol. 1996;34:853–859
  24. Lawrence ER, Arias CA, Duke B, Beste D, Broughton K, Efstratiou A, et al. Evaluation of serotype prediction by cpsA-cpsB gene polymorphism in Streptococcus pneumoniae. J Clin Microbiol. 2000;38:1319–1323
  25. Lawrence ER, Griffiths DB, Martin SA, George RC, Hall LM. Evaluation of semiautomated multiplex PCR assay for determination of Streptococcus pneumoniae serotypes and serogroups. J Clin Microbiol. 2003;41:601–607
  26. Urwin G, Yuan MF, Hall LM, Brown K, Efstratiou A, Feldman RA. Pneumococcal meningitis in the North East Thames Region UK: epidemiology and molecular analysis of isolates. Epidemiol Infect. 1996;117:95–102
  27. Morrison DA, Lacks SA, Guild WR, Hageman JM. Isolation and characterization of three new classes of transformation-deficient mutants of Streptococcus pneumoniae that are defective in DNA transport and genetic recombination. J Bacteriol. 1983;156:281–290
  28. Henderson-Begg SK, Livermore DM, Hall LM. Effect of subinhibitory concentrations of antibiotics on mutation frequency in Streptococcus pneumoniae. J Antimicrob Chemother. 2006;57:849–854
  29. Foster PL. Methods for determining spontaneous mutation rates. Methods Enzymol. 2006;409:195–213
  30. Jones ME, Thomas SM, Rogers A. Luria–Delbruck fluctuation experiments: design and analysis. Genetics. 1994;136:1209–1216
  31. Enright MC, Spratt BG. A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology. 1998;144:3049–3060
  32. Maskell JP. Molecular mechanisms of sulphonamide and trimethoprim resistance in Streptococcus pneumoniae [PhD thesis]. London, UK: Barts and The London School of Medicine and Dentistry, Queen Mary, University of London; 2002.
  33. Denamur E, Bonacorsi S, Giraud A, Duriez P, Hilali F, Amorin C, et al. High frequency of mutator strains among human uropathogenic Escherichia coli isolates. J Bacteriol. 2002;184:605–609
  34. Boe L, Danielsen M, Knudsen S, Petersen JB, Maymann J, Jensen PR. The frequency of mutators in populations of Escherichia coli. Mutat Res. 2000;448:47–55
  35. Funchain P, Yeung A, Stewart JL, Lin R, Slupska MM, Miller JH. The consequences of growth of a mutator strain of Escherichia coli as measured by loss of function among multiple gene targets and loss of fitness. Genetics. 2000;154:959–970

PII: S0924-8579(10)00013-0

doi: 10.1016/j.ijantimicag.2009.12.015

International Journal of Antimicrobial Agents
Volume 35, Issue 4 , Pages 342-346 , April 2010