International Journal of Antimicrobial Agents
Volume 35, Issue 5 , Pages 478-481 , May 2010

Emergence of AcrAB-mediated tigecycline resistance in a clinical isolate of Enterobacter cloacae during ciprofloxacin treatment

  • Michael Hornsey

      Affiliations

    • Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, London NW9 5EQ, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 20 8327 6176; fax: +44 20 8327 6264.
    • ,
  • Matthew J. Ellington

      Affiliations

    • Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, London NW9 5EQ, UK
    • Present address: Clinical Microbiology and Public Health Laboratory, Health Protection Agency, Addenbrookes Hospital, Hills Road, Cambridge CB2 0QQ, UK.
    • ,
  • Michel Doumith

      Affiliations

    • Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, London NW9 5EQ, UK
    • ,
  • Geoff Scott

      Affiliations

    • University College London Hospitals, Windeyer Institute, London W1T 4JF, UK
    • ,
  • David M. Livermore

      Affiliations

    • Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, London NW9 5EQ, UK
    • ,
  • Neil Woodford

      Affiliations

    • Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, London NW9 5EQ, UK

Received 5 January 2010 ,Accepted 6 January 2010.

References 

  1. Reinert RR, Low DE, Rossi F, Zhang X, Wattal C, Dowzicky MJ. Antimicrobial susceptibility among organisms from the Asia/Pacific Rim. Europe and Latin and North America collected as part of TEST and the in vitro activity of tigecycline. J Antimicrob Chemother. 2007;60:1018–1029
  2. Livermore DM, Hope R, Brick G, Lillie M, Reynolds R. Non-susceptibility trends among Enterobacteriaceae from bacteraemias in the UK and Ireland, 2001–06. J Antimicrob Chemother. 2008;62(Suppl. 2):ii41–ii54
  3. Anthony KB, Fishman NO, Linkin DR, Gasink LB, Edelstein PH, Lautenbach E. Clinical and microbiological outcomes of serious infections with multidrug-resistant Gram-negative organisms treated with tigecycline. Clin Infect Dis. 2008;46:567–570
  4. Keeney D, Ruzin A, Bradford PA. RamA, a transcriptional regulator, and AcrAB, an RND-type efflux pump, are associated with decreased susceptibility to tigecycline in Enterobacter cloacae. Microb Drug Resist. 2007;13:1–6
  5. Daurel C, Fiant AL, Brémont S, Courvalin P, Leclercq R. Emergence of an Enterobacter hormaechei strain with reduced susceptibility to tigecycline under tigecycline therapy. Antimicrob Agents Chemother. 2009;53:4953–4954
  6. Andrews JM. BSAC standardized disc susceptibility testing method (version 8). J Antimicrob Chemother. 2009;64:454–489
  7. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. 2nd ed.. Cold Spring Harbour, NY: Cold Spring Harbour Laboratory Press; 1989;
  8. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 2001;25:402–408
  9. Pérez A, Canle D, Latasa C, Poza M, Beceiro A, del Mar Tomas M, et al. Cloning, nucleotide sequencing, and analysis of the AcrAB-TolC efflux pump of Enterobacter cloacae and determination of its involvement in antibiotic resistance in a clinical isolate. Antimicrob Agents Chemother. 2007;51:3247–3253
  10. Chaveroche MK, Ghigo JM, d’Enfert C. A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans. Nucleic Acids Res. 2000;28:E97
  11. Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, et al. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene. 1995;166:175–176

PII: S0924-8579(10)00056-7

doi: 10.1016/j.ijantimicag.2010.01.011

International Journal of Antimicrobial Agents
Volume 35, Issue 5 , Pages 478-481 , May 2010

Article Tools

* Image Usage & Resolution