International Journal of Antimicrobial Agents
Volume 35, Issue 5 , Pages 492-496 , May 2010

Genetic environment of sul genes and characterisation of integrons in Escherichia coli isolates of blood origin in a Spanish hospital

  • Laura Vinué

      Affiliations

    • Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain
    • ,
  • Yolanda Sáenz

      Affiliations

    • Unidad de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja, Logroño, Spain
    • ,
  • Beatriz Rojo-Bezares

      Affiliations

    • Unidad de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja, Logroño, Spain
    • ,
  • Inés Olarte

      Affiliations

    • Laboratorio de Microbiología, Hospital San Pedro, Logroño, Spain
    • ,
  • Esther Undabeitia

      Affiliations

    • Laboratorio de Microbiología, Hospital San Pedro, Logroño, Spain
    • ,
  • Sergio Somalo

      Affiliations

    • Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain
    • ,
  • Myriam Zarazaga

      Affiliations

    • Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain
    • Unidad de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja, Logroño, Spain
    • ,
  • Carmen Torres

      Affiliations

    • Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain
    • Unidad de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja, Logroño, Spain
    • Corresponding Author InformationCorresponding author. Present address: Área de Bioquímica y Biología Molecular, Departamento de Agricultura y Alimentación, Universidad de La Rioja, Madre de Dios 51, 26006 Logroño, Spain. Tel.: +34 941 299 750; fax: +34 941 299 721.

Received 27 October 2009 ,Accepted 12 January 2010.

References 

  1. Partridge SR, Tsafnat G, Coiera E, Iredell JR. Gene cassettes and cassette arrays in mobile resistance integrons. FEMS Microbiol Rev. 2009;33:757–784
  2. Liu J, Keelan P, Bennett PM, Enne VI. Characterization of a novel macrolide efflux gene, mef(B), found linked to sul3 in porcine Escherichia coli. J Antimicrob Chemother. 2009;63:423–426
  3. Soufi L, Abbassi MS, Sáenz Y, Vinué L, Somalo S, Zarazaga M, et al. Prevalence and diversity of integrons and associated resistance genes in Escherichia coli isolates from poultry meat in Tunisia. Foodborne Pathog Dis. 2009;6:1067–1073
  4. Chuanchuen R, Koowatananukul C, Khemtong S. Characterization of class 1 integrons with unusual 3′ conserved region from Salmonella enterica isolates. Southeast Asian J Trop Med Public Health. 2008;39:419–424
  5. Sunde M, Solheim H, Slettemeås JS. Genetic linkage between class 1 integrons with the dfrA12–orfF–aadA2 cassette array and sul3 in Escherichia coli. Vet Microbiol. 2008;130:422–425
  6. Vinué L, Sáenz Y, Somalo S, Escudero E, Moreno MA, Ruiz-Larrea F, et al. Prevalence and diversity of integrons and associated resistance genes in faecal Escherichia coli isolates of healthy humans in Spain. J Antimicrob Chemother. 2008;62:934–937
  7. Antunes P, Machado J, Peixe L. Dissemination of sul3-containing elements linked to class 1 integrons with unusual 3′ conserved sequence region among Salmonella isolates. Antimicrob Agents Chemother. 2007;51:1545–1548
  8. Cocchi S, Grasselli E, Gutacker M, Benagli C, Convert M, Piffaretti JC, et al. Distribution and characterization of integrons in Escherichia coli strains of animal and human origin. FEMS Immunol Med Microbiol. 2007;50:126–132
  9. Sáenz Y, Briñas L, Domínguez E, Ruiz J, Zarazaga M, Vila J, et al. Mechanisms of resistance in multiple-antibiotic-resistant Escherichia coli strains of human, animal, and food origins. Antimicrob Agents Chemother. 2004;48:3996–4001
  10. Sköld O. Sulfonamide resistance: mechanisms and trends. Drug Resist Updat. 2000;3:155–160
  11. Bean DC, Livermore DM, Hall LM. Plasmids imparting sulfonamide resistance in Escherichia coli: implications for persistence. Antimicrob Agents Chemother. 2009;53:1088–1093
  12. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; approved standard. Seventeenth informational supplement. Document M100-S17. Wayne, PA: CLSI; 2007.
  13. Kadlec K, Schwarz S. Analysis and distribution of class 1 and class 2 integrons and associated gene cassettes among Escherichia coli isolates from swine, horses, cats and dogs collected in the BfT-GermVet monitoring study. J Antimicrob Chemother. 2008;62:469–473
  14. Pallecchi L, Lucchetti C, Bartoloni A, Bartalesi F, Mantella A, Gamboa H, et al. Population structure and resistance genes in antibiotic-resistant bacteria from a remote community with minimal antibiotic exposure. Antimicrob Agents Chemother. 2007;51:1179–1184
  15. Haas M, Rak B. Escherichia coli insertion sequence IS150: transposition via circular and linear intermediates. J Bacteriol. 2002;184:5833–5841

PII: S0924-8579(10)00057-9

doi: 10.1016/j.ijantimicag.2010.01.012

International Journal of Antimicrobial Agents
Volume 35, Issue 5 , Pages 492-496 , May 2010

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