International Journal of Antimicrobial Agents
Volume 35, Issue 6 , Pages 584-586 , June 2010

First description of Klebsiella pneumoniae clinical isolates carrying both qnrA and qnrB genes in Portugal

  • Sónia Ferreira

      Affiliations

    • University of Aveiro, Department of Biology, CESAM, 3810-193 Aveiro, Portugal
    • School of Medicine, Department of Medical Microbiology, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    • Hospital Infante D. Pedro EPE, 3810 Aveiro, Portugal
  • ,
  • Mark Toleman

      Affiliations

    • School of Medicine, Department of Medical Microbiology, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
  • ,
  • Elmano Ramalheira

      Affiliations

    • Hospital Infante D. Pedro EPE, 3810 Aveiro, Portugal
  • ,
  • Gabriela J. Da Silva

      Affiliations

    • Center of Pharmaceutical Sciences, University of Coimbra, Health Sciences Campus, Faculty of Pharmacy, Department of Microbiology, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
  • ,
  • Timothy Walsh

      Affiliations

    • School of Medicine, Department of Medical Microbiology, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
  • ,
  • Sónia Mendo

      Affiliations

    • University of Aveiro, Department of Biology, CESAM, 3810-193 Aveiro, Portugal
    • Corresponding Author InformationCorresponding author. Present address: CESAM & Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal. Tel.: +351 234 370 970; fax: +351 234 372 587.

Received 12 November 2009 ,Accepted 14 January 2010.

References 

  1. Nordmann P, Poirel L. Emergence of plasmid-mediated resistance to quinolones in Enterobacteriaceae. J Antimicrob Chemother. 2005;56:463–469
  2. Wang A, Yang Y, Lu Q, Wang Y, Chen Y, Deng L, et al. Occurrence of qnr-positive clinical isolates in Klebsiella pneumoniae producing ESBL or AmpC-type β-lactamase from five pediatric hospitals in China. FEMS Microbiol Lett. 2008;283:112–116
  3. Cavaco LM, Hansen DS, Friis-Moller A, Aarestrup FM, Hasman H, Frimodt-Moller N. First detection of plasmid-mediated quinolone resistance (qnrA and qnrS) in Escherichia coli strains isolated from humans in Scandinavia. J Antimicrob Chemother. 2007;59:804–805
  4. Martínez-Martínez L, Pascual A, Jacoby GA. Quinolone resistance from a transferable plasmid. Lancet. 1998;351:797–799
  5. Park Y-J, Yu JK, Lee S, Oh E-J, Woo G-J. Prevalence and diversity of qnr alleles in AmpC-producing Enterobacter cloacae, Enterobacter aerogenes, Citrobacter freundii and Serratia marcescens: a multicentre study from Korea. J Antimicrob Chemother. 2007;60:868–871
  6. Robicsek A, Sahm DF, Strahilevitz J, Jacoby GA, Hooper DC. Broader distribution of plasmid-mediated quinolone resistance in the United States. Antimicrob Agents Chemother. 2005;49:3001–3003
  7. Lascols C, Podglajen I, Verdet C, Gautier V, Gutmann L, Soussy CJ, et al. A plasmid-borne Shewanella algae gene, qnrA3, and its possible transfer in vivo between Kluyvera ascorbata and Klebsiella pneumoniae. J Bacteriol. 2008;190:5217–5223
  8. Garnier F, Raked N, Gassama A, Denis F, Ploy M-C. Genetic environment of quinolone resistance gene qnrB2 in a complex sul1-type integron in the newly described Salmonella enterica serovar Keurmassar. Antimicrob Agents Chemother. 2006;50:3200–3202
  9. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Seventeenth informational supplement. Document M100-S17. Wayne, PA: CLSI; 2007.
  10. Barlow RS, Pemberton JM, Desmarchelier PM, Gobius KS. Isolation and characterization of integron-containing bacteria without antibiotic selection. Antimicrob Agents Chemother. 2004;48:838–842
  11. Wu JJ, Ko WC, Tsai SH, Yan JJ. Prevalence of plasmid-mediated quinolone resistance determinants QnrA, QnrB, and QnrS among clinical isolates of Enterobacter cloacae in a Taiwanese hospital. Antimicrob Agents Chemother. 2007;51:1223–1227
  12. Henriques IS, Fonseca F, Alves A, Saavedra MJ, Correia A. Occurrence and diversity of integrons and β-lactamase genes among ampicillin-resistant isolates from estuarine waters. Res Microbiol. 2006;157:938–947
  13. Machado E, Coque TM, Cantón R, Baquero F, Sousa JC, Peixe L, et al. Dissemination in Portugal of CTX-M-15-, OXA-1-, and TEM-1-producing Enterobacteriaceae strains containing the aac(6)-Ib-cr gene, which encodes an aminoglycoside- and fluoroquinolone-modifying enzyme. Antimicrob Agents Chemother. 2006;50:3220–3221
  14. Cordeiro NF, Robino L, Medina J, Seija V, Bado I, García V, et al. Ciprofloxacin-resistant enterobacteria harboring the aac(6)-Ib-cr variant isolated from feces of inpatients in an intensive care unit in Uruguay. Antimicrob Agents Chemother. 2008;52:806–807
  15. Kim S-Y, Park Y-J, Yu JK, Kim YS, Han K. Prevalence and characteristics of aac(6)-Ib-cr in AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens: a multicenter study from Korea. Diagn Microbiol Infect Dis. 2009;63:314–318
  16. Pai H, Seo M-R, Choi TY. Association of qnrB determinants and production of extended-spectrum β-lactamases or plasmid-mediated AmpC β-lactamases in clinical isolates of Klebsiella pneumoniae. Antimicrob Agents Chemother. 2007;51:366–368

 This work was presented in part at the 48th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) and the 46th Annual Meeting of the Infectious Diseases Society of America (IDSA), 25–28 October 2008, Washington DC.

PII: S0924-8579(10)00064-6

doi: 10.1016/j.ijantimicag.2010.01.019

International Journal of Antimicrobial Agents
Volume 35, Issue 6 , Pages 584-586 , June 2010