International Journal of Antimicrobial Agents
Volume 35, Issue 6 , Pages 593-598 , June 2010

Comparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae

  • Fiona Walsh

      Affiliations

    • Department of Clinical Microbiology, Sir Patrick Dun Translational Research Laboratory, School of Medicine, University of Dublin, Trinity College, St James's Hospital Campus, Dublin 8, Ireland
    • These authors contributed equally to this work.
    • Corresponding Author InformationCorresponding author. Tel.: +353 1 896 3620; fax: +353 1 896 2131.
    • ,
  • Niamh M. Cooke

      Affiliations

    • Department of Clinical Microbiology, Sir Patrick Dun Translational Research Laboratory, School of Medicine, University of Dublin, Trinity College, St James's Hospital Campus, Dublin 8, Ireland
    • These authors contributed equally to this work.
    • ,
  • Stephen G. Smith

      Affiliations

    • Department of Clinical Microbiology, Sir Patrick Dun Translational Research Laboratory, School of Medicine, University of Dublin, Trinity College, St James's Hospital Campus, Dublin 8, Ireland
    • ,
  • Gary P. Moran

      Affiliations

    • Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, University of Dublin, Trinity College, Dublin 2, Ireland
    • ,
  • Fiona J. Cooke

      Affiliations

    • Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    • ,
  • Alasdair Ivens

      Affiliations

    • Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    • ,
  • John Wain

      Affiliations

    • Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    • ,
  • Thomas R. Rogers

      Affiliations

    • Department of Clinical Microbiology, Sir Patrick Dun Translational Research Laboratory, School of Medicine, University of Dublin, Trinity College, St James's Hospital Campus, Dublin 8, Ireland

Received 23 December 2009 ,Accepted 10 February 2010.

References 

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  4. Bruant G, Maynard C, Bekal S, Gaucher I, Masson L, Brousseau R, et al. Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli. Appl Environ Microbiol. 2006;72:3780–3784
  5. Cleven BE, Palka-Santini M, Gielen J, Meembor S, Kronke M, Krut O. Identification and characterization of bacterial pathogens causing bloodstream infections by DNA microarray. J Clin Microbiol. 2006;44:2389–2397
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  7. Yu X, Susa M, Weile J, Knabbe C, Schmid RD, Bachmann TT. Rapid and sensitive detection of fluoroquinolone-resistant Escherichia coli from urine samples using a genotyping DNA microarray. Int J Med Microbiol. 2007;297:417–429
  8. Cooke FJ, Wain J, Fookes M, Ivens A, Thomson N, Brown DJ, et al. Prophage sequences defining hot spots of genome variation in Salmonella enterica serovar Typhimurium can be used to discriminate between field isolates. J Clin Microbiol. 2007;45:2590–2598
  9. Moran G, Stokes C, Thewes S, Hube B, Coleman DC, Sullivan D. Comparative genomics using Candida albicans DNA microarrays reveals absence and divergence of virulence-associated genes in Candida dubliniensis. Microbiology. 2004;150:3363–3382
  10. Bukh AS, Schonheyder HC, Emmersen JM, Sogaard M, Bastholm S, Roslev P. Escherichia coli phylogenetic groups are associated with site of infection and level of antibiotic resistance in community-acquired bacteraemia: a 10 year population-based study in Denmark. J Antimicrob Chemother. 2009;64:163–168
  11. Cooke NM, Smith SG, Kelleher M, Rogers TR. Major differences exist in frequencies of virulence factors and multidrug resistance between community and nosocomial Escherichia coli bloodstream isolates. J Clin Microbiol 2010 Jan 27 [Epub ahead of print].
  12. Johnson JR, Kuskowski MA, O’Bryan TT, Maslow JN. Epidemiological correlates of virulence genotype and phylogenetic background among Escherichia coli blood isolates from adults with diverse-source bacteremia. J Infect Dis. 2002;185:1439–1447
  13. Naas T, Namdari F, Reglier-Poupet H, Poyart C, Nordmann P. Panresistant extended-spectrum β-lactamase SHV-5-producing Acinetobacter baumannii from New York City. J Antimicrob Chemother. 2007;60:1174–1176
  14. Uda A, Tanabayashi K, Fujita O, Hotta A, Yamamoto Y, Yamada A. Comparison of whole genome amplification methods for detecting pathogenic bacterial genomic DNA using microarray. Jpn J Infect Dis. 2007;60:355–361

PII: S0924-8579(10)00085-3

doi: 10.1016/j.ijantimicag.2010.02.011

International Journal of Antimicrobial Agents
Volume 35, Issue 6 , Pages 593-598 , June 2010

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