International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 234-238 , September 2010

Assessment of the activity of RND-type multidrug efflux pumps in Pseudomonas aeruginosa using tetraphenylphosphonium ions

  • Rimantas Daugelavičius

      Affiliations

    • Department of Biochemistry and Biophysics, Vilnius University, Lithuania
    • Department of Biochemistry and Biotechnologies, Vytautas Magnus University, Kaunas, Lithuania
    • These authors contributed equally to this work.
    • Corresponding Author InformationCorresponding author. Present address: Department of Biochemistry and Biotechnologies, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos 8, LT-44404 Kaunas, Lithuania. Tel.: +370 37 327 917; fax: +370 37 327 916.
    • ,
  • Andrius Buivydas

      Affiliations

    • Department of Biochemistry and Biophysics, Vilnius University, Lithuania
    • Department of Biological Sciences and Institute of Biotechnology, University of Helsinki, Finland
    • These authors contributed equally to this work.
    • ,
  • Ana Senčilo

      Affiliations

    • Department of Biochemistry and Biophysics, Vilnius University, Lithuania
    • Department of Biological Sciences and Institute of Biotechnology, University of Helsinki, Finland
    • ,
  • Dennis H. Bamford

      Affiliations

    • Department of Biological Sciences and Institute of Biotechnology, University of Helsinki, Finland

Received 6 February 2010 ,Accepted 24 March 2010.

References 

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  3. Babayan A, Nikaido H. In Pseudomonas aeruginosa ethidium bromide does not induce its own degradation or the assembly of pumps involved in its efflux. Biochem Biophys Res Commun. 2004;324:1065–1068
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  6. Daugelavicius R, Cvirkaite V, Gaidelyte A, Bakiene E, Gabrenaite-Verkhovskaya R, Bamford DH. Penetration of enveloped double-stranded RNA bacteriophages phi13 and phi6 into Pseudomonas syringae cells. J Virol. 2005;79:5017–5026
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  10. Gros P, Talbot F, Tang-Wai D, Bibi E, Kaback HR. Lipophilic cations: a group of model substrates for the multidrug-resistance transporter. Biochemistry. 1992;31:1992–1998
  11. Lomovskaya O, Warren MS, Lee A, Galazzo J, Fronko R, Lee M, et al. Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy. Antimicrob Agents Chemother. 2001;45:105–116
  12. Sambrook J, Russell DW. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 2001;
  13. Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc. 2008;3:163–175
  14. Daugelavicius R, Gaidelyte A, Cvirkaite-Krupovic V, Bamford DH. On-line monitoring of changes in host cell physiology during the one-step growth cycle of Bacillus phage Bam35. J Microbiol Methods. 2007;69:174–179
  15. Pannek S, Higgins PG, Steinke P, Jonas D, Akova M, Bohnert JA, et al. Multidrug efflux inhibition in Acinetobacter baumannii: comparison between 1-(1-naphthylmethyl)-piperazine and phenyl-arginine-β-naphthylamide. J Antimicrob Chemother. 2006;57:970–974

PII: S0924-8579(10)00162-7

doi: 10.1016/j.ijantimicag.2010.03.028

International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 234-238 , September 2010

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