International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 211-215 , September 2010

Ambroxol interferes with Pseudomonas aeruginosa quorum sensing

  • Qi Lu

      Affiliations

    • Department of Neonatology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China
  • ,
  • Jialin Yu

      Affiliations

    • Department of Neonatology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China
    • Corresponding Author InformationCorresponding author. Tel.: +86 23 6363 4756.
  • ,
  • Xiqiang Yang

      Affiliations

    • Department of Clinical Immunology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China
    • Corresponding Author InformationCorresponding author. Tel.: +86 23 6362 4479.
  • ,
  • Jiarong Wang

      Affiliations

    • Department of Neonatology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China
  • ,
  • Lijia Wang

      Affiliations

    • Department of Clinical Immunology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China
  • ,
  • Yayin Lin

      Affiliations

    • Department of Neonatology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China
  • ,
  • Lihua Lin

      Affiliations

    • Department of Neonatology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China

Received 10 October 2009 ,Accepted 6 May 2010.

References 

  1. Davies JC. Pseudomonas aeruginosa in cystic fibrosis: pathogenesis and persistence. Paediatr Respir Rev. 2002;3:128–134
  2. Lyczak JB, Cannon C, Pier GB. Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist. Microbes Infect. 2000;2:1051–1060
  3. Pier GB. Pseudomonas aeruginosa: a key problem in cystic fibrosis. ASM News. 1998;6:339–347
  4. Bergmans DC, Bonten MJ, Gaillard CA, van Tiel FH, van der Geest S, de Leeuw PW, et al. Indications for antibiotic use in ICU patients: a 1-year prospective surveillance. J Antimicrob Chemother. 1997;39:527–535
  5. Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science. 1999;284:1318–1322
  6. Smith RS, Harris SG, Phipps R, Iglewski B. The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)homoserine lactone contributes to virulence and induces inflammation in vivo. J Bacteriol. 2002;184:1132–1139
  7. Rumbaugh KP, Griswold JA, Hamood AN. The role of quorum sensing in the in vivo virulence of Pseudomonas aeruginosa. Microbes Infect. 2000;2:1721–1731
  8. Hentzer M, Wu H, Andersen JB, Riedel K, Rasmussen TB, Bagge N, et al. Attenuation of Pseudomonas aeruginosa virulence by quorum-sensing inhibitors. EMBO J. 2003;22:3803–3815
  9. Wu H, Song Z, Hentzner M, Andersen JB, Molin S, Givskov M, et al. Synthetic furanones inhibit quorum-sensing and enhance bacterial clearance in Pseudomonas aeruginosa lung infection in mice. J Antimicrob Chemother. 2004;53:1054–1061
  10. Tateda K, Ishii Y, Yamaguchi K. Quorum-sensing system of Pseudomonas aeruginosa—a novel target for antibacterial agents. Nippon Saikingaku Zasshi. 2004;59:543–549
  11. Yamada T, Takemura Y, Niisato N, Mitsuyama E, Iwasaki Y, Marunaka Y. Action of N-acylated ambroxol derivatives on secretion of chloride ions in human airway epithelia. Biochem Biophys Res Commun. 2009;380:586–590
  12. Stetinová V, Herout V, Kvetina J. In vitro and in vivo antioxidant activity of ambroxol. Clin Exp Med. 2004;4:152–158
  13. Li F, Yu J, Wan Z, Bai D. Effects of ambroxol on alginate of mature Pseudomonas aeruginosa biofilms. Curr Microbiol. 2008;57:1–7
  14. Wagner VE, Koberstein JT, Bryers JD. Protein and bacterial fouling characteristics of peptide and antibody decorated surfaces of PEG-poly(acrylic acid) co-polymers. Biomaterials. 2004;25:2247–2263
  15. Strathmann M, Wingender J, Flemming HC. Application of fluorescently labelled lectins for the visualization and biochemical characterization of polysaccharides in biofilms of Pseudomonas aeruginosa. J Microbiol Methods. 2002;50:237–248
  16. Beyenal H, Donovan C, Lewandowski Z, Harkin G. Three-dimensional biofilm structure quantification. J Microbiol Methods. 2004;59:395–413
  17. Boulos L, Prévost M, Barbeau B, Coallier J, Desjardins R. LIVE/DEAD® BacLight™: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water. J Microbiol Methods. 1999;37:77–86
  18. Takayama S, McDonald JC, Ostuni E, Liang MN, Kenis PJ, Ismagilov RF, et al. Patterning cells and their environments using multiple laminar fluid flows in capillary networks. Proc Natl Acad Sci USA. 1999;96:5545–5548
  19. Davies DG, Parsek MR, Pearson JP, Iglewski BH, Costerton JW, Greenberg EP. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science. 1998;280:295–298
  20. Mittal R, Sharma S, Chhibber S, Harjai K. Contribution of quorum-sensing systems to virulence of Pseudomonas aeruginosa in an experimental pyelonephritis model. J Microbiol Immunol Infect. 2006;39:302–309
  21. Banin E, Vasil ML, Greenberg EP. Iron and Pseudomonas aeruginosa biofilm formation. Proc Natl Acad Sci USA. 2005;102:11076–11081
  22. Davey ME, Caiazza NC, O’Toole GA. Rhamnolipid surfactant production affects biofilm architecture in Pseudomonas aeruginosa PAO1. J Bacteriol. 2003;185:1027–1036
  23. Lequette Y, Greenberg EP. Timing and localization of rhamnolipid synthesis gene expression in Pseudomonas aeruginosa biofilms. J Bacteriol. 2005;187:37–44
  24. Yoon SS, Hennigan RF, Hilliard GM, Ochsner UA, Parvatiyar K, Kamani MC, et al. Pseudomonas aeruginosa anaerobic respiration in biofilms: relationships to cystic fibrosis pathogenesis. Dev Cell. 2002;3:593–603
  25. Al Laham N, Rohde H, Sander G, Fischer A, Hussain M, Heilmann C, et al. Augmented expression of polysaccharide intercellular adhesin in a defined Staphylococcus epidermidis mutant with the small-colony-variant phenotype. J Bacteriol. 2007;189:4494–5012
  26. Labbate M, Zhu H, Thung L, Bandara R, Larsen MR, Willcox MD, et al. Quorum-sensing regulation of adhesion in Serratia marcescens MG1 is surface dependent. J Bacteriol. 2007;189:2702–2711
  27. Kuehn M, Hausner M, Bungartz HJ, Wagner M, Wilderer PA, Wuertz S. Automated confocal laser scanning microscopy and semiautomated image processing for analysis of biofilms. Appl Environ Microbiol. 1998;64:4115–4127
  28. Heydorn A, Ersbøll B, Kato J, Hentzer M, Parsek MR, Tolker-Nielsen T, et al. Statistical analysis of Pseudomonas aeruginosa biofilm development: impact of mutations in genes involved in twitching motility, cell-to-cell signaling, and stationary-phase sigma factor expression. Appl Environ Microbiol. 2002;68:2008–2017
  29. Heydorn A, Nielsen AT, Hentzer M, Sternberg C, Givskov M, Ersbøll BK, et al. Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology. 2000;146:2395–2407
  30. Singh PK, Schaefer AL, Parsek MR, Moninger TO, Welsh MJ, Greenberg EP. Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms. Nature. 2000;407:762–764

PII: S0924-8579(10)00225-6

doi: 10.1016/j.ijantimicag.2010.05.007

International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 211-215 , September 2010