International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 255-258 , September 2010

Emergence of clinical strains of Mycoplasma genitalium harbouring alterations in ParC associated with fluoroquinolone resistance

  • Yasushi Shimada

      Affiliations

    • Department of Urology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
    • Research and Development, Narita R&D Department, POCT Group, Mitsubishi Chemical Medience Corporation, 4-2-8 Shibaura, Minato-Ku, Tokyo 108-8559, Japan
    • ,
  • Takashi Deguchi

      Affiliations

    • Department of Urology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81 58 230 6338; fax: +81 58 230 6339.
    • ,
  • Keita Nakane

      Affiliations

    • Department of Urology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
    • ,
  • Takako Masue

      Affiliations

    • Department of Urology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
    • ,
  • Mitsuru Yasuda

      Affiliations

    • Department of Urology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
    • ,
  • Shigeaki Yokoi

      Affiliations

    • Department of Urology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
    • ,
  • Shin-ichi Ito

      Affiliations

    • Department of Urology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
    • ,
  • Masahiro Nakano

      Affiliations

    • Department of Urology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
    • ,
  • Shin Ito

      Affiliations

    • iClinic, 7-19-26 Naga-Machi, Taihaku-Ku, Sendai 982-0011, Japan
    • ,
  • Hiroaki Ishiko

      Affiliations

    • Host Defense Laboratory, Mitsubishi Chemical Medience Corporation, 4-2-8 Shibaura, Minato-Ku, Tokyo 108-8559, Japan

Received 19 April 2010 ,Accepted 20 May 2010.

References 

  1. Tully JG, Taylor-Robinson D, Cole RM, Rose DL. A newly discovered mycoplasma in the human urogenital tract. Lancet. 1981;1:1288–1291
  2. Mena LA, Mroczkowski TF, Nsuami M, Martin DH. A randomized comparison of azithromycin and doxycycline for the treatment of Mycoplasma genitalium-positive urethritis in men. Clin Infect Dis. 2009;48:1649–1654
  3. Yasuda M, Maeda S, Deguchi T. In vitro activity of fluoroquinolones against Mycoplasma genitalium and their bacteriological efficacy for treatment of M. genitalium-positive nongonococcal urethritis in men. Clin Infect Dis. 2005;41:1357–1359
  4. Jernberg E, Moghaddam A, Moi H. Azithromycin and moxifloxacin for microbiological cure of Mycoplasma genitalium infection: an open study. Int J STD AIDS. 2008;19:676–679
  5. Bébéar CM, Renaudin J, Charron A, Renaudin H, de Barbeyrac B, Schaeverbeke T, et al. Mutations in the gyrA, parC, and parE genes associated with fluoroquinolone resistance in clinical isolates of Mycoplasma hominis. Antimicrob Agents Chemother. 1999;43:954–956
  6. Gruson D, Pereyre S, Renaudin H, Charron A, Bébéar C, Bébéar CM. In vitro development of resistance to six and four fluoroquinolones in Mycoplasma pneumoniae and Mycoplasma hominis, respectively. Antimicrob Agents Chemother. 2005;49:1190–1193
  7. Lysnyansky I, Gerchman I, Perk S, Levisohn S. Molecular characterization and typing of enrofloxacin-resistant clinical isolates of Mycoplasma gallisepticum. Avian Dis. 2008;52:685–689
  8. Hirose K, Kawasaki Y, Kotani K, Abiko K, Sato H. Characterization of a point mutation in the parC gene of Mycoplasma bovirhinis associated with fluoroquinolone resistance. J Vet Med B Infect Dis Vet Public Health. 2004;51:169–175
  9. Le Carrou J, Reinhardt AK, Kempf I, Gautier-Bouchardon AV. Persistence of Mycoplasma synoviae in hens after two enrofloxacin treatments and detection of mutations in the parC gene. Vet Res. 2006;37:145–154
  10. Vicca J, Maes D, Stakenborg T, Butaye P, Minion F, Peeters J, et al. Resistance mechanism against fluoroquinolones in Mycoplasma hyopneumoniae field isolates. Microb Drug Resist. 2007;13:166–170
  11. Lysnyansky I, Mikula I, Gerchman I, Levisohn S. Rapid detection of a point mutation in the parC gene associated with decreased susceptibility to fluoroquinolones in Mycoplasma bovis. Antimicrob Agents Chemother. 2009;53:4911–4914
  12. Beeton ML, Chalker VJ, Kotecha S, Spiller OB. Comparison of full gyrA, gyrB, parC and parE gene sequences between all Ureaplasma parvum and Ureaplasma urealyticum serovars to separate true fluoroquinolone antibiotic resistance mutations from non-resistance polymorphism. J Antimicrob Chemother. 2009;64:529–538
  13. Yoshida H, Bogaki M, Nakamura M, Nakamura S. Quinolone resistance-determining region in the DNA gyrase gyrA gene of Escherichia coli. Antimicrob Agents Chemother. 1990;34:1271–1272
  14. Yoshida T, Maeda S, Deguchi T, Miyazawa T, Ishiko H. Rapid detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum organisms in genitourinary samples by PCR-microtiter plate hybridization assay. J Clin Microbiol. 2003;41:1850–1855
  15. Deguchi T, Maeda S, Tamaki M, Yoshida T, Ishiko H, Ito M, et al. Analysis of the gyrA and parC genes of Mycoplasma genitalium detected in first-pass urine of men with non-gonococcal urethritis before and after fluoroquinolone treatment. J Antimicrob Chemother. 2001;48:742–744

PII: S0924-8579(10)00229-3

doi: 10.1016/j.ijantimicag.2010.05.011

International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 255-258 , September 2010

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