International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 259-263, September 2010

Characteristics of clinical isolates of Acinetobacter genomospecies 10 carrying two different metallo-β-lactamases☆

  • Kyungwon Lee

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Chang Ki Kim

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Seong Geun Hong

      Affiliations

    • Department of Laboratory Medicine, College of Medicine, Cha University, Seongnam, South Korea
    • ,
  • Jonghyeon Choi

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Sungwook Song

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Eunmi Koh

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Dongeun Yong

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Seok Hoon Jeong

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Jong Hwa Yum

      Affiliations

    • Department of Clinical Laboratory Science, Dong-eui University, Busan, South Korea
    • ,
  • Jean-Denis Docquier

      Affiliations

    • Department of Molecular Biology, Section of Microbiology, University of Siena, Siena, Italy
    • ,
  • Gian Maria Rossolini

      Affiliations

    • Department of Molecular Biology, Section of Microbiology, University of Siena, Siena, Italy
    • ,
  • Yunsop Chong

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • Corresponding Author InformationCorresponding author. Present address: Department of Laboratory Medicine, Yonsei University College of Medicine, 250 Seongsanro, Seodaemungu, Seoul, South Korea. Tel.: +82 2 2228 2446; fax: +82 2 313 0908.

Received 9 March 2010; accepted 31 May 2010. published online 05 July 2010.

Abstract 

Acquired metallo-β-lactamase (MBL) production is an important mechanism of carbapenem resistance. To our knowledge, carriage of two different MBLs has not been described previously in Acinetobacter spp. We present the characteristics of five Acinetobacter isolates carrying two different MBL genes. The species of all five Acinetobacter isolates with two different MBL genes were Acinetobacter genomospecies 10, and blaIMP-1, blaVIM-2 and blaSIM-1 may coexist in this species. Minimum inhibitory concentrations (MICs) of imipenem for all five isolates with two different MBLs were ≥32μg/mL, whilst those for two segregants that lost both MBLs were 0.5μg/mL. The presence of MBL gene-carrying integrons with identical structures suggested the easily transferable nature of the elements, whilst instability of the MBL genes indicated potentially erroneous phenotypic and genetic characterisation.

Keywords: MBL genes, Acinetobacter genomospecies 10, Imipenem resistance

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☆ Parts of these results were presented at the 46th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), 27–30 September 2006, San Francisco, CA (C2-413) and at the 47th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), 17–22 September 2007, Chicago, IL (C2-1362).

PII: S0924-8579(10)00237-2

doi:10.1016/j.ijantimicag.2010.05.018

International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 259-263, September 2010

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