International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 259-263 , September 2010

Characteristics of clinical isolates of Acinetobacter genomospecies 10 carrying two different metallo-β-lactamases

  • Kyungwon Lee

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Chang Ki Kim

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Seong Geun Hong

      Affiliations

    • Department of Laboratory Medicine, College of Medicine, Cha University, Seongnam, South Korea
    • ,
  • Jonghyeon Choi

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Sungwook Song

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Eunmi Koh

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Dongeun Yong

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Seok Hoon Jeong

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • ,
  • Jong Hwa Yum

      Affiliations

    • Department of Clinical Laboratory Science, Dong-eui University, Busan, South Korea
    • ,
  • Jean-Denis Docquier

      Affiliations

    • Department of Molecular Biology, Section of Microbiology, University of Siena, Siena, Italy
    • ,
  • Gian Maria Rossolini

      Affiliations

    • Department of Molecular Biology, Section of Microbiology, University of Siena, Siena, Italy
    • ,
  • Yunsop Chong

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
    • Corresponding Author InformationCorresponding author. Present address: Department of Laboratory Medicine, Yonsei University College of Medicine, 250 Seongsanro, Seodaemungu, Seoul, South Korea. Tel.: +82 2 2228 2446; fax: +82 2 313 0908.

Received 9 March 2010 ,Accepted 31 May 2010.

References 

  1. Bush K. New β-lactamases in Gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy. Clin Infect Dis. 2001;32:1085–1089
  2. Lee M-F, Peng C-F, Hsu HJ, Chen Y-H. Molecular characterisation of the metallo-β-lactamase genes in imipenem-resistant Gram-negative bacteria from a university hospital in southern Taiwan. Int J Antimicrob Agents. 2008;32:475–480
  3. Lee K, Yum JH, Yong D, Lee HM, Kim HD, Docquier J-D, et al. Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. Antimicrob Agents Chemother. 2005;49:4485–4491
  4. Lim YM, Shin KS, Kim J. Distinct antimicrobial resistance patterns and antimicrobial resistance-harboring genes according to genomic species of Acinetobacter isolates. J Clin Microbiol. 2007;45:902–905
  5. Yan JJ, Ko WC, Tsai SH, Wu HM, Wu JJ. Outbreak of infection with multidrug-resistant Klebsiella pneumoniae carrying blaIMP-8 in a university medical center in Taiwan. J Clin Microbiol. 2001;39:4433–4439
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  7. Lee K, Yong D, Yum JH, Lim YS, Bolmstrom A, Qwarnstrom A, et al. Evaluation of Etest MBL for detection of blaIMP-1 and blaVIM-2 allele-positive clinical isolates of Pseudomonas spp. and Acinetobacter spp.. J Clin Microbiol. 2005;43:942–944
  8. Lim YS, Yong D, Yum JH, Lee K, Chong Y. Difficulties in the detection of resistance gene and the determination of imipenem resistance of the metallo-β-lactamase-producing Gram-negative bacilli. In: 42nd Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), 27–30 September 2002, San Diego, CA. Washington, DC: ASM Press; 2002;p. 139;[Abstr. D 530]
  9. Turton JF, Kaufmann ME, Coelho JM, Warner M, Pike R, Pitt TL. Detection and typing of integrons in epidemic strains of Acinetobacter baumannii found in the United Kingdom. J Clin Microbiol. 2005;43:3074–3082
  10. Lee K, Kim M-N, Choi TY, Cho S-E, Lee S, Whang DH, et al. Wide dissemination of OXA-type carbapenemases in clinical Acinetobacter spp. isolates from South Korea. Int J Antimicrob Agents. 2009;33:520–524
  11. Chang HC, Wei YF, Dijkshoorn L, Vaneechoutte M, Tang CT, Chang TC. Species-level identification of isolates of the Acinetobacter calcoaceticus–Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region. J Clin Microbiol. 2005;43:1632–1639
  12. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Nineteenth informational supplement. Document M100-S19. Wayne, PA: CLSI; 2009.
  13. Tsakris A, Ikonomidis A, Poulou A, Spanakis N, Vrizas D, Diomidous M, et al. Clusters of imipenem-resistant Acinetobacter baumannii clones producing different carbapenemases in an intensive care unit. Clin Microbiol Infect. 2008;14:588–594
  14. Gombac F, Riccio ML, Rossolini GM, Lagatolla C, Tonin E, Monti-Bragadin C, et al. Molecular characterization of integrons in epidemiologically unrelated clinical isolates of Acinetobacter baumannii from Italian hospitals reveals a limited diversity of gene cassette arrays. Antimicrob Agents Chemother. 2002;46:3665–3668
  15. Barton BM, Harding GP, Zuccarelli AJ. A general method for detecting and sizing large plasmids. Anal Biochem. 1995;226:235–240

☆ Parts of these results were presented at the 46th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), 27–30 September 2006, San Francisco, CA (C2-413) and at the 47th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), 17–22 September 2007, Chicago, IL (C2-1362).

PII: S0924-8579(10)00237-2

doi: 10.1016/j.ijantimicag.2010.05.018

International Journal of Antimicrobial Agents
Volume 36, Issue 3 , Pages 259-263 , September 2010

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